Biochemical Composition and Anticancer Effect of Different Seaweed Species (In-vitro and In-vivo Studies)

T H Ranahewa

Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lank

A. D. Premarathna

Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka

R.M. K.K. Wijesundara

Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka

V. Wijewardana

Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka; Department of Atomic Energy Agency (IAEA) Vienna, Austria

A. P. Jayasooriya

Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka; Department of Basic Veterinary Sciences, Faculty of Medical Sciences, University of West Indies, Trinidad and Tobago, West Indies

R.P. V.J. Rajapakse

Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka


Copyright © 2020 T H Ranahewa, A. D. Premarathna, R.M. K.K. Wijesundara, V. Wijewardana, A. P. Jayasooriya, R.P. V.J. Rajapakse. Published by Nan Yang Academy of Sciences Pte. Ltd.

Creative Commons LicenseThis is an open access article under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License.


Seaweed is an enormous resource comprised with natural bioactive compounds with several therapeutic effects including anticancer activity. In this context, the biochemical composition of seaweed plays a major role. Many biochemical compounds isolated from seaweed, fractions of seaweed and crude extracts has revealed ability of seaweed to fight against several cancer types. In this contrast seaweed extracts inhibit cancer cell growth and proliferation by inducing apoptosis and by inhibiting metastasis activity. In this review, biochemical and anticancer properties of seaweeds are discussed and this will provide the basic information to develop a novel chemotherapeutic drug to challenge the cancers.

Keywords: Seaweed, Biochemical, Bioactive, Anticancer, Apoptosis


[1] Abirami, R.G., Kowsalya, S. Anticancer activity of methanolic and aqueous extracts of Ulva fasciata in albino mice. International Journal of Pharmacy and Pharmaceutical Sciences, 2012, 4(2): 681-684.

[2] Afolayan, A.F. et al. Fucoxanthin, tetraprenylated toluquinone and toluhydroquinone metabolites from Sargassum heterophyllum inhibit the in vitro growth of the malaria parasite Plasmodium falciparum. 2008, 63: 848-852.

[3] Aisa, Y. et al. Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways. American Journal of Hematology, 2005, 78: 7-14.

[4] Arulvasu, C., Shiva Shakthi, S., Rangasamy, V., Hemavathi, M. In-vitro antioxidant and anticancer activity of selected seaweeds of kovalam coast, tamilnadu. 2014: 2347-3207.

[5] Chojnacka, K., Kim, S. Introduction of marine algae extracts”, In S. Kim and K. Chojnacka, eds. Marine Algae Extracts: Processes, Products, and Applications. First Edition ed. Wiley: VCH Verlag GmbH and Co. KGaA, 2015.

[6] Dhargalkar, V., Kavlekar, D. Seaweeds- a field manual, X N Verlecar and V Rathod, Eds. Dona Paula, Goa: National Institute of Oceanography, 2004.

[7] Ermakova, S. et al. Fucoidans from brown seaweeds Sargassum hornery, Eclonia cava, Costaria costata: structural characteristics and anticancer activity. Appl Biochem Biotechnol, 2011, 164: 841-850.

[8] Faried, A. et al. Anticancer effects of gallic acid isolated from Indonesian herbal medicine, Phaleria macrocarpa (Scheff.) Boerl, on human cancer cell lines. International journal of oncology, 2007, 30: 605-613.

[9] Farooqi, A.A., Butt, G., Razzaq, Z. Algae extracts and methyl jasmonate anti-cancer activities in prostate cancer: Choreographers of the dance macabre. 2012, 12(50).

[10] Fedorov, S.N. et al. Dactylone inhibits epidermal growth factor-induced transformation and phenotype expression of human cancer cells and induces G1-S arrest and apoptosis. 2007, 67: 5914-5921.

[11] Gaudet, M.M. et al. Fruits, vegetables, and micronutrients in relation to breast cancer modified by menopause and hormone receptor status. Cancer Epidemiol. Biomarkers, 2004, 13: 1485-1494.

[12] Gomes, D.L. et al. Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells. 2015, 20: 6573-91.

[13] Gupta, S., Abu-Ghannam, N. Bioactive potential and possible health effects of edible brown seaweeds. Trends in Food Science & Technology, 2011, 22: 315-26.

[14] Holdt, S.L., Kraan, S. Bioactive compounds in seaweed: Functional food applications. Journal of Applied Phycology, 2011, 23: 543-597.

[15] Hosokawa, M. et al. Apoptosis inducing effect of Fucoxanthin on human leukemia cell line HL-60. Food sci. technolol. res, 1999, 5(3): 243-46.

[16] Hyun, J. et al. Apoptosis inducing activity of fucoidan in HCT-15 colon carcinoma cells. 2009, 32(10): 1760-1764.

[17] Isnansetyo, A. et al. Cytotoxicity of fucoidan from three tropical brown algae against Breast and colon cancer cell lines. Pharmacogn Journal, 2017, 9(1): 14-20.

[18] Jo, E.H. et al. Modulations of the Bcl-2/Bax family were involved in the chemopreventive effects of licorice root (Glycyrrhiza uralensis Fisch) in MCF-7 human breast cancer cell. J. Agric. Food Chem, 2004, 52: 1715-1719.

[19] Kazłowska, K., Lin, T.V., Chang, S., Tsai, G. In vitro and in vivo anticancer effects of sterol fraction from red algae Porphyra dentata. Natural Bioactives and Phytochemicals Serve in Cancer Treatment and Prevention, 2013.

[20] Kim, E.J., Park, S.Y., Lee, J.Y., Park, J.H. Fucoidan present in brown algae induces apoptosis of human colon cancer cells. BMC Gastroenterology, 2010, 10(96): 1471-1482.

[21] Lakmal, H.H. et al. Anticancer and antioxidant effects of selected Sri Lankan marine algae. J.Natn.Sci.Foundation Sri Lanka, 2014, 42(4): 315-23.

[22] Lau, T.Y., Vitta, D.F., Chew, S.Y., Yong, W.T. Antiproliferative potential of extracts from kappaphycus seaweeds on HeLa cancer cell lines. Sains Malaysiana, 2014, 43(12): 1895-1900.

[23] Liu, G. et al. A novel polysaccharide from Sargassum integerrimum inducesapoptosis in A549 cells and preventsangiogensis in vitro and in vivo. Scientific reports, 2016, 6: 26722-26734.

[24] Manivannan, K. et al. Biochemical composition of seaweeds from mandapam coastal regions along southeast coast of india. American-Eurasian Journal of Botany, 2008, 1(2): 32-37.

[25] Miyata, M. et al. Anti-obesity effect on rodents of the traditional Japanese food, tororokombu, shaved Laminaria. 2009, 73: 2326-2328.

[26] Moussavou, G. et al. Anticancer effects of different seaweeds on human colon and breast cancers. Marine drugs, 2014, 12: 4898-4911.

[27] Namvar, F. et al. Polyphenol-rich seaweed (Eucheuma cottonii) extract suppresses breast tumour via hormone modulation and apoptosis induction. Food Chemistry, 2012, 130: 376-382.

[28] Namvar, F. et al. Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum). 2013.

[29] Narasimhan, M., Pavithra, S.K., Krishnan, V., Chandrasekaran, M. In vitro analysis of antioxidant, antimicrobial and antiproliferative activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata Extracts”, Jundishapur Journal of Natural Pharmaceutical Products, 2013, 8(4): 151-159.

[30] Narayan, B., Miyashita, K., Hosakawa, M. Physiological effects of eicosapentaenoicnoic acid (EPA) and docosahexaenoic acid (DHA)-A review. Journal Food Reviews International, 2006, 22: 291-307.

[31] Park, H.K., Kim, J., Nam, T.J. Induction of apoptosis and regulation of ErbB signaling by laminarin in HT-29 human colon cancer cells. 2013, 32: 291-295.

[32] Philchenkov, A. et al. Sensitization of human malignant lymphoid cells to etoposide by fucoidan, a brown seaweed polysaccharide. 2007, 29(3): 181-185.

[33] Premarathna, A.D. et al. Wound healing properties of aqueous extracts of Sargassum illicifolium: An in vitro assay. Wound Medicine, 2019, 24: 1-7.

[34] Rajapakse, N., Kim, S. Advances in food and nutrition research. In Nutritional and Digestive Health Benefits of Seaweed, 2011.

[35] Rocha, D.H., Seca, A.M., Pinto, D.C. Seaweed secondary metabolites in vitro and in vivo anticancer activity. 2018, 16(11): 410.

[36] Ryu, M.J. et al. The green algae Ulva fasciata Delile extract induces apoptotic cell death in human colon cancer cells”, In Vitro Cell.Dev.Biol.-Animal, 2013, 49: 74-81.

[37] Wijesekara, I., Pangestuti, R., Kim, S. Biological activities and potential health benefits of sulfated polysaccharidesderived from marine algae. Carbohydrate Polymers, 2011, 84: 14-21.

[38] Yan, X., Chuda, Y., Suzuki, M., Nagata, T. Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. 2011, 63: 605-607.

[39] Yang, L. et al. Fucoidan derived from Undaria pinnatifada induces apoptosis in human hepatocellular carcinoma SMMC 7721 cells via the ROS mediated mitochondrial pathway. 2013, 11: 1961-1976.

[40] Yaoxian, W. et al. Emodin induces apoptosis of human cervical cancer hela cells via intrinsic mitochondrial and extrinsic death receptor pathway. Cancer Cell International, 2013, 13(71).

[41] Ye, H., Zhou, C., Liu, J., Zeng, X. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chemistry, 2008: 428-432.

[42] Zandi, K. et al. Anticancer activity of Sargassum oligocystum water extract against human cancer cell lines. European Review for Medical and Pharmacological Sciences, 2010 14: 669-73.

[43] Zandi, K. et al. In vitro antitumor activity of Gracilaria corticata (a red alga) against Jurkat and molt-4 human cancer cell lines. African Journal of Biotechnology, 2010, 9(40): 6787-90.

[44] Zhang, Z. et al. Potential chemoprevention effect of dietary fucoxanthin on urinary bladder cancer EJ-1 cell line. Oncology Reports, 2008, 20: 1099-1103.

[45] Zhuang, C., Itoh, H., Mizuno, T., Ito, H. Antitumor active fucoidan from the brown seaweed, Umitoranoo (Sargassum thunbergii). 2014, 22(41)